RUBCN

rubicon autophagy regulator
General Information
Name rubicon autophagy regulator
Alias
  • run domain Beclin-1-interacting and cysteine-rich domain-containing protein
  • RUN and cysteine rich domain containing beclin 1 interacting protein
  • RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein
  • baron
  • beclin-1 associated RUN domain containing protein
  • rundataxin
Synonyms rubicon rundataxin KIAA0226 RUBICON SCAR15
Gene_family MicroRNA protein coding host genes|PIK3C3 complex subunits
organism Homo sapiens
entrez_id 9711
location 3q29
transcript_count 9
exon_count 24
Cross References
Location
by NCBI GRCh38.p14
    Summary
      Entrez The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
      nextProt Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation (PubMed:20974968, PubMed:21062745).
    Interactions
    NCBI
    A A.Source B B.DB B.Source Description PubMed
    RUBCN BioGRID RNF40 GeneID BioGRID Cross-Linking-MS (XL-MS)
    RUBCN BioGRID S GeneID BioGRID Affinity Capture-MS
    RUBCN BioGRID RNF34 GeneID BioGRID Affinity Capture-Western; Co-localization; Reconstituted Complex
    RUBCN BioGRID ZFYVE21 GeneID BioGRID Affinity Capture-Western; Co-localization; Reconstituted Complex
    RUBCN BioGRID PYCARD GeneID BioGRID Reconstituted Complex
    RUBCN BioGRID RAB5A GeneID BioGRID Affinity Capture-Western; Co-localization
    RUBCN BioGRID FLII GeneID BioGRID Affinity Capture-Western
    RUBCN BioGRID CASP1 GeneID BioGRID Affinity Capture-Western
    RUBCN BioGRID BECN1 GeneID BioGRID Affinity Capture-MS; Affinity Capture-Western; Co-fractionation; Co-purification
    RUBCN BioGRID UVRAG GeneID BioGRID Affinity Capture-MS; Affinity Capture-Western
    RUBCN BioGRID RAB7A GeneID BioGRID Reconstituted Complex
    RUBCN BioGRID ATG9A GeneID BioGRID Proximity Label-MS
    RUBCN BioGRID YWHAZ GeneID BioGRID Affinity Capture-MS
    RUBCN BioGRID PIK3C3 GeneID BioGRID Affinity Capture-MS; Affinity Capture-Western
    RUBCN BioGRID BECN2 GeneID BioGRID Affinity Capture-Western
    RUBCN BioGRID STXBP4 GeneID BioGRID Affinity Capture-MS
    RUBCN BioGRID SPATA33 GeneID BioGRID Affinity Capture-MS
    RUBCN BioGRID HOOK3 GeneID BioGRID Affinity Capture-MS
    RUBCN BioGRID HOOK1 GeneID BioGRID Affinity Capture-MS
    RUBCN BioGRID PIK3R4 GeneID BioGRID Affinity Capture-MS; Affinity Capture-Western
    RUBCN BioGRID ZFYVE26 GeneID BioGRID Affinity Capture-MS
    RUBCN BioGRID NUBP2 GeneID BioGRID Affinity Capture-MS
    RUBCN BioGRID IQCB1 GeneID BioGRID Affinity Capture-MS
    RUBCN BioGRID YWHAH GeneID BioGRID Affinity Capture-MS
    RUBCN BioGRID YWHAG GeneID BioGRID Affinity Capture-MS
    RUBCN BioGRID RIGI GeneID BioGRID Affinity Capture-RNA
    RUBCN BioGRID RAB9A GeneID BioGRID Proximity Label-MS
    RUBCN BioGRID LAMP2 GeneID BioGRID Proximity Label-MS
    RUBCN BioGRID PRC1 GeneID BioGRID Affinity Capture-MS
    RUBCN BioGRID LOC102724334 GeneID BioGRID Proximity Label-MS
    RUBCN BioGRID H2BC18 GeneID BioGRID Proximity Label-MS
    RUBCN BioGRID PDIA4 GeneID BioGRID Proximity Label-MS
    RUBCN BioGRID ATG14 GeneID BioGRID Affinity Capture-Western
    RUBCN BioGRID FAM9B GeneID BioGRID Two-hybrid
    RUBCN BioGRID TCEANC GeneID BioGRID Two-hybrid
    RUBCN BioGRID ZNF648 GeneID BioGRID Two-hybrid
    RUBCN BioGRID FAM161A GeneID BioGRID Two-hybrid
    RUBCN BioGRID ENKD1 GeneID BioGRID Two-hybrid
    RUBCN BioGRID ZNF250 GeneID BioGRID Two-hybrid
    RUBCN BioGRID NME7 GeneID BioGRID Two-hybrid
    RUBCN BioGRID ANKRD11 GeneID BioGRID Two-hybrid
    RUBCN BioGRID BRD1 GeneID BioGRID Two-hybrid
    RUBCN BioGRID VEZF1 GeneID BioGRID Two-hybrid
    RUBCN BioGRID DPF2 GeneID BioGRID Two-hybrid
    RUBCN BioGRID GAS8 GeneID BioGRID Two-hybrid
    RUBCN BioGRID BYSL GeneID BioGRID Two-hybrid
    RUBCN BioGRID APEX1 GeneID BioGRID Affinity Capture-RNA
    RUBCN BioGRID MTOR GeneID BioGRID Affinity Capture-Western
    RUBCN BioGRID EGFR GeneID BioGRID Affinity Capture-Western
    RUBCN BioGRID IKBKG GeneID BioGRID Affinity Capture-Western; Co-localization
    RUBCN BioGRID EXOC8 GeneID BioGRID Affinity Capture-Western
    RUBCN BioGRID EXOC2 GeneID BioGRID Affinity Capture-Western
    RUBCN BioGRID EXOC1 GeneID BioGRID Affinity Capture-Western; Two-hybrid
    RUBCN BioGRID YWHAE GeneID BioGRID Affinity Capture-MS
    RUBCN Protein SMAD9 GeneID HPRD None
    StringDB
    A B experiments database textmining combined score hadb nhadb
    KIAA0226 KAT5 0 0 148 166 2 1
    KIAA0226 AMBRA1 0 0 637 701 2 1
    KIAA0226 WIPI1 0 0 459 543 2 1
    KIAA0226 GABARAP 0 0 392 485 2 1
    KIAA0226 ATG7 0 0 556 573 2 1
    KIAA0226 PAQR3 0 0 124 262 2 1
    KIAA0226 TBC1D14 0 0 150 257 2 1
    KIAA0226 ULK1 0 0 517 528 2 1
    KIAA0226 STX7 0 0 142 266 2 1
    KIAA0226 MAP1LC3B 0 0 402 445 2 1
    KIAA0226 ATG10 0 0 468 482 2 1
    KIAA0226 ATG5 0 0 557 568 2 1
    KIAA0226 CALCOCO2 0 0 319 319 2 1
    KIAA0226 HMGB1 0 0 128 237 2 1
    KIAA0226 OPTN 0 0 247 246 2 1
    KIAA0226 CTSB 0 0 143 262 2 1
    KIAA0226 SMCR8 0 0 0 168 2 1
    KIAA0226 BECN1 898 0 989 998 2 1
    KIAA0226 LRRK2 0 0 194 213 2 1
    KIAA0226 GRID2 0 0 608 616 2 1
    KIAA0226 ATG16L1 0 0 509 525 2 1
    KIAA0226 PLEKHM1 0 0 294 159 2 1
    KIAA0226 ATG101 0 0 455 545 2 1
    KIAA0226 ATG4D 0 0 205 474 2 1
    KIAA0226 ATG12 0 0 507 528 2 1
    KIAA0226 RB1 0 0 0 161 2 1
    KIAA0226 ZFYVE1 0 0 455 455 2 1
    KIAA0226 BNIP3L 0 0 294 294 2 1
    KIAA0226 TSC2 0 0 173 192 2 1
    KIAA0226 STX17 0 0 414 435 2 1
    KIAA0226 NRBF2 0 0 466 466 2 1
    KIAA0226 ATG2B 0 0 270 291 2 1
    KIAA0226 ATG3 0 0 501 511 2 1
    KIAA0226 ATG4C 0 0 231 302 2 1
    KIAA0226 EVA1A 0 0 128 219 2 1
    KIAA0226 PINK1 0 0 181 181 2 1
    KIAA0226 VMP1 0 0 336 336 2 1
    KIAA0226 TMEM74 0 0 189 206 2 1
    KIAA0226 AKT1 0 0 205 217 2 1
    KIAA0226 PPP1R15A 0 0 457 457 2 1
    KIAA0226 WIPI2 0 0 504 586 2 1
    KIAA0226 NBR1 0 0 371 472 2 1
    KIAA0226 VPS11 0 0 210 259 2 1
    KIAA0226 UVRAG 875 800 987 999 2 1
    KIAA0226 APOL1 0 0 171 190 2 1
    KIAA0226 WDFY3 0 0 166 228 2 1
    KIAA0226 WDR45 0 0 167 182 2 1
    KIAA0226 RAB7A 800 0 606 931 2 1
    KIAA0226 DRAM1 0 0 241 535 2 1
    KIAA0226 RUFY4 0 0 142 167 2 1
    KIAA0226 ATG4B 0 0 310 344 2 1
    KIAA0226 EPG5 0 0 162 198 2 1
    KIAA0226 STX8 0 0 0 286 2 1
    KIAA0226 CHMP4B 0 0 0 188 2 1
    KIAA0226 STBD1 0 0 81 174 2 1
    KIAA0226 ATG2A 0 0 245 261 2 1
    KIAA0226 PTPN2 0 0 0 150 2 1
    KIAA0226 DRAM2 0 0 164 216 2 1
    KIAA0226 ATG16L2 0 0 150 157 2 1
    KIAA0226 MAP1LC3C 0 0 252 298 2 1
    KIAA0226 MAPK8 0 0 202 221 2 1
    KIAA0226 TBC1D5 0 0 95 164 2 1
    KIAA0226 RPTOR 0 0 264 286 2 1
    KIAA0226 ITPR1 0 0 161 216 2 1
    KIAA0226 SH3GLB1 0 0 629 659 2 1
    KIAA0226 SNAP29 0 0 295 321 2 1
    KIAA0226 ULK2 0 0 413 431 2 1
    KIAA0226 DAPK1 0 0 281 404 2 1
    KIAA0226 LAMP2 0 0 241 256 2 1
    KIAA0226 FUNDC1 0 0 160 170 2 1
    KIAA0226 EIF2AK2 0 0 433 446 2 1
    KIAA0226 RAB24 0 0 142 597 2 1
    KIAA0226 ATG9A 0 0 240 318 2 1
    KIAA0226 GABARAPL1 0 0 374 470 2 1
    KIAA0226 TFEB 0 0 254 254 2 1
    KIAA0226 CLEC16A 0 0 204 271 2 1
    KIAA0226 ATG13 0 0 519 549 2 1
    KIAA0226 BNIP3 0 0 170 169 2 1
    KIAA0226 SQSTM1 0 0 503 581 2 1
    KIAA0226 MFN2 0 0 136 176 2 1
    KIAA0226 MAP1LC3A 0 0 283 327 2 1
    KIAA0226 RAB5A 0 0 453 469 2 1
    KIAA0226 NEDD4 0 0 139 157 2 1
    KIAA0226 PIK3C3 873 0 908 988 2 1
    KIAA0226 CHMP2B 0 0 140 173 2 1
    KIAA0226 MTMR3 0 0 234 264 2 1
    KIAA0226 MTOR 270 0 335 512 2 1
    KIAA0226 ATG14 270 0 850 893 2 1
    KIAA0226 GABARAPL2 0 0 391 455 2 1
    KIAA0226 PIK3R4 486 0 886 941 2 1
    KIAA0226 BCL2L1 0 0 336 351 2 1
    KIAA0226 FYCO1 0 0 170 186 2 1
    KIAA0226 GLIPR2 0 0 177 177 2 1
    KIAA0226 RICTOR 0 0 170 169 2 1
    KIAA0226 IRGM 0 0 294 310 2 1
    KIAA0226 TM9SF1 0 0 169 519 2 1
    KIAA0226 RB1CC1 0 0 564 575 2 1
    KIAA0226 TAX1BP1 0 0 181 181 2 1
    KIAA0226 LAMP1 0 0 415 425 2 1
    KIAA0226 RAB9A 0 0 0 163 2 1
    KIAA0226 HDAC6 0 0 107 152 2 1
    KIAA0226 VAMP8 0 0 235 248 2 1
    Ontologies
    ID Category Term Effect Evidence PubMed
    GO:0141039 Molecular Function phosphatidylinositol 3-kinase inhibitor activity enables IDA
    GO:1901981 Molecular Function phosphatidylinositol phosphate binding enables IBA
    GO:0005515 Molecular Function protein binding enables IPI
    GO:0006914 Biological Process autophagy involved_in IEA
    GO:0002376 Biological Process immune system process involved_in IEA
    GO:0071985 Biological Process multivesicular body sorting pathway involved_in TAS
    GO:1901097 Biological Process negative regulation of autophagosome maturation involved_in IBA
    GO:1901097 Biological Process negative regulation of autophagosome maturation involved_in IMP
    GO:1901097 Biological Process negative regulation of autophagosome maturation involved_in TAS
    GO:0010507 Biological Process negative regulation of autophagy involved_in IMP
    GO:0045806 Biological Process negative regulation of endocytosis involved_in IBA
    GO:0045806 Biological Process negative regulation of endocytosis involved_in IMP
    GO:0051898 Biological Process negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction involved_in IDA
    GO:0006909 Biological Process phagocytosis involved_in IEA
    GO:0005794 Cellular Component Golgi apparatus NOT colocalizes_with IDA
    GO:0005829 Cellular Component cytosol located_in IDA
    GO:0005769 Cellular Component early endosome is_active_in IBA
    GO:0005769 Cellular Component early endosome located_in IDA
    GO:0043231 Cellular Component intracellular membrane-bounded organelle located_in IDA
    GO:0005770 Cellular Component late endosome is_active_in IBA
    GO:0005770 Cellular Component late endosome located_in IDA
    GO:0005764 Cellular Component lysosome located_in IEA
    GO:0005654 Cellular Component nucleoplasm located_in IDA
    Pathways
    Name DB ID
    Transcripts
    Accession Version MolecularType Name NCBI Comments
    XM_006713831 5 mRNA transcript variant X6 NC_000003 Reference
    NM_001145642 5 mRNA transcript variant 1 NC_000003 Reference
    XM_047449268 1 mRNA transcript variant X3 NC_000003 Reference
    XM_017007543 2 mRNA transcript variant X5 NC_000003 Reference
    NM_014687 4 mRNA transcript variant 2 NC_000003 Reference
    XM_006713828 4 mRNA transcript variant X2 NC_000003 Reference
    NM_001346873 2 mRNA transcript variant 3 NC_000003 Reference
    XM_005269374 4 mRNA transcript variant X4 NC_000003 Reference
    XM_006713827 4 mRNA transcript variant X1 NC_000003 Reference